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業績リストはこちら。

Peer-Reviewed Papers (*, Corresponding Author; †, Equal Contribution)

  1. Rong Chen, Jiayuan Ji, Yujie Chen, Yasuyuki Takemura, Yuan Liu, Kengo Kubota, Haiyuan Ma, Yu-You Li*. 2019. Successful operation performance and syntrophic micro-granule in partial nitritation and anammox reactor treating low-strength ammonia wastewater. Water Research. 155: 288-299.
  2. Lu Li, Yu Qin, Zhe Kong, Jing Wu, Kengo Kubota, Yu-You Li*. 2019. Characterization of microbial community and main functional groups of prokaryotes in thermophilic anaerobic co-digestion of food waste and paper waste. Science of The Total Environment. 652: 709-717.
  3. Kong, Z., L. Li, R. Kurihara, K. Kubota, Y.-Y. Li*. 2018. Anaerobic treatment of N, N-dimethylformamide-containing wastewater by co-culturing two sources of inoculum. Water Research. 139: 228-239.
  4. Lu, X., J. Ni, G. Zhen, K. Kubota, Y.-Y. Li*. 2018. Response of morphology and microbial community structure of granules to influent COD/SO42 – ratios in an upflow anaerobic sludge blanket (UASB) reactor treating starch wastewater. Bioresource Technology. 256: 456-465.
  5. Nomoto, N., M. Hatamoto, Y. Hirakata, M. Ali, K. Jayaswal, A. Iguchi, T. Okubo, M. Takahashi, K. Kubota, T. Tagawa, S. Uemura, T. Yamaguchi*, H. Harada. Defining microbial community composition and seasonal variation in a sewage treatment plant in India using a down-flow hanging sponge reactor. Applied Microbiology and Biotechnology. 102(10): 4381-4392.
  6. Oshiki, M.*, T. Miura, S. Kazama, T. Segawa, S. Ishii, M. Hatamoto, T. Yamaguchi, K. Kubota, A. Iguchi, T. Tagawa, T. Okubo, S. Uemura, H. Harada, N. Kobayashi, N. Araki, D. Sano. 2018. Microfluidic PCR amplification and MiSeq amplicon sequencing techniques for high-throughput detection and genotyping of human pathogenic RNA viruses in human feces, sewage, and oysters. Frontiers in Microbiology, section Virology. 9: 830
  7. Nomoto, N., M. Ali, K. Jayaswal, A. Iguchi, M. Hatamoto, T. Okubo, M. Takahashi, K. Kubota, T. Tagawa, S. Uemura, T. Yamaguchi*, H. Harada. 2018. Characteristics of DO, organic matter, and ammonium profile for practical-scale DHS reactor under various organic load and temperature conditions. Environmental Technology. 39(7): 907-916.
  8. Nomoto, N., M. Hatamoto, M. Ali, K. Jayaswal, A. Iguchi, T. Okubo, M. Takahashi, K. Kubota, T. Tagawa, S. Uemura, T. Yamaguchi*, H. Harada. 2018. Characterization of sludge properties for sewage treatment in a practical-scale down-flow hanging sponge reactor: oxygen consumption and removal of organic matter, ammonium, and sulfur. Water Science and Technology. 77(3): 608-616.
  9. Qin, Y., A. Higashimori, L.-J. Wu, T. Hojo, K. Kubota, Y.-Y. Li*. 2017. Phase separation and microbial distribution in the hyperthermophilic-mesophilic-type temperature-phased anaerobic digestion (TPAD) of waste activated sludge (WAS). Bioresource Technology. 245(December): 401-410.
  10. Okubo, T., A. Iguchi, M. Takahashi, K. Kubota, S. Uemura, H. Harada. 2017. Treatment performance of practical-scale down-flow hanging sponge reactor using sixth-generation hard sponge media. Desalination and Water Treatment. 91: 48-54. (October)
  11. Matsubayashi, M., Y. Shimada, Y.-Y. Li, H. Harada, K. Kubota*. 2017. Phylogenetic diversity and in situ detection of eukaryotes in anaerobic sludge digesters. PLoS ONE. 12(3): e0172888.
  12. Kobayashi, N., M. Oshiki*, T. Ito, T. Segawa, M. Hatamoto, T. Kato, T. Yamaguchi, K. Kubota, M. Takahashi, A. Iguchi, T. Tagawa, T. Okubo, S. Uemura, H. Harada, T. Motoyama, N. Araki, D. Sano. 2017. Removal of human pathogenic viruses in a down-flow hanging sponge (DHS) reactor treating municipal wastewater and health risks associated with utilization of the effluent for agricultural irrigation. Water Research. 110(March): 389-398.
  13. Lu, X., G. Zhen*, J. Ni, K. Kubota, Y.-Y. Li*. 2017. Sulfidogenesis process to strengthen re-granulation for biodegradation of methanolic wastewater and microorganisms evolution in an UASB reactor. Water Research. 108(January): 137-150.
  14. Okubo, T.*, K. Kubota, T. Yamaguchi, S. Uemura, and H. Harada. 2016. Development of a new non-aeration-based sewage treatment technology: Performance evaluation of a full-scale down-flow hanging sponge reactor employing third-generation sponge carriers. Water Research. 102(October): 138-146.
  15. Hirakata, Y., M. Oshiki*, K. Kuroda, M. Hatamoto, K. Kubota, T. Yamaguchi, H. Harada, and N. Araki. 2016. Effects of predation by protists on prokaryotic community function, structure, and diversity in anaerobic granular sludge. Microbes and Environments. 31(3): 279-287.
  16. Uemura, S.*, T. Okubo, K. Maeno, M. Takahashi, K. Kubota, and H. Harada, 2016. Evaluation of water distribution and oxygen mass transfer in sponge support media for a down-flow hanging sponge reactor. International Journal of Environmental Research. 10(2): 265-272.
  17. Lu, X., G. Zhen, J. Ni, T. Hojo, K. Kubota, and Y.-Y. Li*. 2016. Effect of influent COD/SO42- ratios on biodegradation behaviors of starch wastewater in an upflow anaerobic sludge blanket (UASB) reactor. Bioresource Technology. 214(August): 175-183.
  18. Zhang, Y., Q. Niu, H. Ma, S. He, K. Kubota, and Y.-Y. Li*. 2016. Long-term operation performance and variation of substrate tolerance ability in an anammox attached film expanded bed (AAFEB) reactor. Bioresource Technology. 211(July): 31-40.
  19. Wu, L.-J., A. Higashimori, Y. Qin, T. Hojo, K. Kubota, and Y.-Y. Li*. 2016. Comparison of hyper-thermophilic-mesophilic two-stage with single-stage mesophilic anaerobic digestion of waste activated sludge: Process performance and microbial community analysis. Chemical Engineering Journal. 290(April): 290-301.
  20. Wu, L.-J., A. Higashimori, Y. Qin, T. Hojo, K. Kubota, and Y.-Y. Li*. 2016. Upgrading of mesophilic anaerobic digestion of waste activated sludge by thermophilic pre-fermentation and recycle: Process performance and microbial community analysis. Fuel. 169(April): 7-14.
  21. Hirakata, Y., M. Oshiki*, K. Kuroda, M. Hatamoto, K. Kubota, T. Yamaguchi, H. Harada, and N. Araki. 2015. Identification and detection of prokaryotic symbionts in the ciliate Metopus from anaerobic granular sludge. Microbes and Environments. 30(4): 335-338.
  22. Lu, X., G. Zhen, M. Chen, K. Kubota, and Y.-Y. Li*. 2015. Biocatalysis conversion of methanol to methane in an upflow anaerobic sludge blanket (UASB) reactor: Long-term performance and inherent deficiencies. Bioresource Technology. 198(December): 691-700.
  23. Niu, Q., K. Kubota, W. Qiao, Z. Jing, Y. Zhang, Y.Y. Li*. 2015. Effect of ammonia inhibition on microbial community dynamic and process functional resilience in mesophilic methane fermentation of chicken manure. Journal of Chemical Technology & Biotechnology. 90(12): 2161-2169.
  24. Elreedy, A.*, A. Tawfik, K. Kubota, Y. Shimada, and H. Harada. 2015. Hythane (H2 + CH4) production from petrochemical wastewater containing mono-ethylene glycol via stepped anaerobic baffled reactor. International Biodeterioration & Biodegradation. 105(November): 252-261.
  25. Niu, Q., T. Kobayashi, Y. Takemura, K. Kubota, and Y.-Y. Li*. 2015. Evaluation of functional microbial community’s difference in full-scale and lab-scale anaerobic digesters feeding with different organic solid waste: Effects of substrate and operation factors. Bioresource Technology. 193(October): 110-118.
  26. Yamaguchi, T.*, B.M. Fuchs, R. Amann, S. Kawakami, K. Kubota, M. Hatamoto, and T. Yamaguchi. 2015. Rapid and sensitive identification of marine bacteria by an improved in situ DNA hybridization chain reaction (quickHCR-FISH). Systematic and Applied Microbiology. 38(6): 400-405.
  27. Niu, Q., Y. Takemura, K. Kubota, and Y.-Y. Li*. 2015. Comparing mesophilic and thermophilic anaerobic digestion of chicken manure: microbial community dynamics and process resilience. Waste Management. 43(September): 114-122.
  28. Yamaguchi, T., S. Kawakami*, M. Hatamoto, H. Imachi, M. Takahashi, N. Araki, T. Yamaguchi, and K. Kubota. 2015. In situ DNA-hybridization chain reaction (HCR): a facilitated in situ HCR system for the detection of environmental microorganisms. Environmental Microbiology. 17(7): 2532-2541.
  29. Lu, X.*, G. Zhen*, A.L. Estrada, M. Chen, J. Ni, T. Hojo, K. Kubota, and Y.-Y. Li*. 2015. Operation performance and granule characterization of upflow anaerobic sludge blanket (UASB) reactor treating wastewater with starch as the sole carbon source. Bioresource Technology. 180(March): 264-273.
  30. Matsunaga, K., K. Kubota*, and H. Harada. 2014. Molecular diversity of eukaryotes in municipal wastewater treatment processes as revealed by 18S rRNA gene analysis. Microbes and Environments. 29(4): 401-407.
  31. Nobu, M., H. Tamaki, K. Kubota, and W.-T. Liu*. 2014. Metagenomic characterization of ‘Candidatus Defluviicoccus tetraformis strain TFO71’, a tetrad-forming organism, predominant in an anaerobic–aerobic membrane bioreactor with deteriorated biological phosphorus removal. Environmental Microbiology. 16(9): 2739-2751.
  32. Bao, Z., T. Okubo, K. Kubota, Y. Kasahara, H. Tsurumaru, M. Anda, S. Ikeda, and K. Minamisawa*. 2014. Metaproteomic identification of diazotrophic methanotrophs and their localization in root tissues of field-grown rice plants. Applied and Environmental Microbiology. 80(16): 5043-5052.
  33. Kubota, K.†*, Y. Morono†*, M. Ito, T. Terada, S. Itezono, H. Harada, and F. Inagaki. 2014. Gold-ISH: A nano-size gold particle-based phylogenetic identification compatible with NanoSIMS. Systematic and Applied Microbiology. 37(4): 261-266.
  34. Kubota, K.*, M. Hayashi, K. Matsunaga, A. Iguchi, A. Ohashi, Y.-Y. Li, T. Yamaguchi, and H. Harada. 2014. Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage. Bioresource Technology. 151(January): 144-150.
  35. Fang, F., Y. Mu, G.-P. Sheng, H.-Q. Yu*, Y.-Y. Li, K. Kubota, and H. Harada. 2013. Kinetic analysis on gaseous and aqueous product formation by mixed anaerobic hydrogen-producing cultures. International Journal of Hydrogen Energy. 38(35): 15590-15597.
  36. Onodera, T.†*, K. Matsunaga†, K. Kubota, R. Taniguchi, H. Harada, K. Syutsubo, T. Okubo, S. Uemura, N. Araki, M. Yamada, M. Yamauchi, and T. Yamaguchi*. 2013. Characterization of the retained sludge in a down-flow hanging sponge (DHS) reactor with emphasis on its low excess sludge production. Bioresource Technology. 136(May): 169-175.
  37. Kawakami, S., T. Hasegawa, H. Imachi, T. Yamaguchi, H. Harada, A. Ohashi, and K. Kubota*. Detection of single-copy functional genes in prokaryotic cells by two-pass TSA-FISH with polynucleotide probes. Journal of Microbiological Methods. 88(2): 218-223.
  38. Kobayashi, T., Y. Y. Li*, K. Kubota, H. Harada, T. Maeda, and H. Q. Yu. Characterization of sulfide-oxidizing microbial mats developed inside a full-scale anaerobic digester employing biological desulfurization. Applied Microbiology and Biotechnology. 93(2): 847-857.
  39. Dong, F., Q.-B. Zhao, W.-W. Li*, G.-P. Sheng, J.-B. Zhao, Y. Tang, H.-Q. Yu*, K. Kubota, Y.-Y. Li, and H. Harada. 2011. Novel online monitoring and alert system for anaerobic digestion reactors. Environmental Science and Technology. 45(20): 9093-9100.
  40. Yoochatchaval, W.*, S. Kumakura, D. Tanikawa, T. Yamaguchi, M. F. M. Yunus, S. S. Chen, K. Kubota, H. Harada, and K. Syutsubo*. 2011. Anaerobic degradation of palm oil mill effluent (POME). Water Science and Technology. 64(10): 2001-2008.
  41. Kawakami, S., K. Kubota*, H. Imachi, T. Yamaguchi, H. Harada, and A. Ohashi. 2010. Detection of single copy genes by two-pass tyramide signal amplification fluorescence in situ hybridization (two-pass TSA-FISH) with single oligonucleotide probes. Microbes and Environments. 25(1): 15-21.
  42. Kobayashi, T., D. Yasuda, Y. Y. Li*, K. Kubota, H. Harada, and H. Q. Yu. 2009. Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge. Bioresource Technology. 100(21): 4981-4988.
  43. Akutsu, Y., Y. Y. Li*, M. Tandukar, K. Kubota, and H. Harada. 2008. Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch. International Journal of Hydrogen Energy. 33(22): 6541-6548.
  44. Kubota, K., H. Imachi, S. Kawakami, K. Nakamura, H. Harada, and A. Ohashi*. 2008. Evaluation of enzymatic cell treatments for application of CARD-FISH to methanogens. Journal of Microbiological Methods. 72(1): 54-59.
  45. Kubota, K., A. Ohashi*, H. Imachi, and H. Harada. 2006. Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes. Applied and Environmental Microbiology. 72(8): 5311-5317.
  46. Kubota, K., A. Ohashi*, H. Imachi, and H. Harada. 2006. Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA-FISH). Journal of Microbiological Methods. 66(3): 521-528.

Review Papers (*, Corresponding Author)

  1. Hatamoto, M., T. Okubo, K. Kubota, and T. Yamaguchi*. 2018. Characterization of down-flow hanging sponge reactors with regard to structure, process function, and microbial community compositions. Applied Microbiology and Biotechnology. 102(24): 10345-10352.
  2. Kubota, K.*. 2013. CARD-FISH for environmental microorganisms: technical advancement and future applications. Microbes and Environments. 28(1): 3-12.

Book Chapter

  1. Yamaguchi, T.* and K. Kubota. 2017. Visualization of Microorganisms in Bioprocesses. in Optimization and Applicability of Bioprocesses, H. Purohit, V. Kalia, A. Vaidya, A. Khardenavis (eds)., Springer, Singapore (Published online 03 January 2018)
  2. Kubota, K., Y. Morono, and M. Ito. 2017. Nanoscale Secondary-Ion Mass Spectrometry Imaging of Biological Structures. in Encyclopedia of Analytical Chemistry, R.A. Meyers (eds.), John Wiley: Chichester. (Published March 25 2017)
  3. Kubota, K., and S. Kawakami. Protocol for in situ detection of functional genes of microorganisms by two-pass TSA-FISH. in Hydrocarbon and Lipid Microbiology Protocols, T.J. McGenity, K.N. Timmis, B. Nogales (eds.), Springer Protocols Handbooks (2016) 131–144, Springer-Verlag Berlin Heidelberg (Published online: 04 April 2015)

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